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Enteroendocrine L-cells secrete GLP-1 in response to various nutrients after food intake, releasing it into the bloodstream
75 g of protein lysates were heated for 10 min at 95 C in Laemmli 6X SDS sample buffer (Boston BioProducts, # BP-111R) with 5% 2-mercaptoethanol (VWR Life Science #M131-100ml) and then ran on 420% Criterion TGX pre-cast gels (Bio-Rad #5671093)
This multi-receptor activation could provide superior efficacy compared to current single or dual agonist therapies
Specifically, it is a fragment representing the active portion of natural GHRH
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