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Lees studies are smaller than most rigorous clinical trials, ranging in size from two to 16 study participants, and dont include comparison groups
However, the lack of oxygen and substrates generally keeps ROS generation relatively lower compared to the subsequent phase
The samples destined for GC/MS analysis were re-dried under vacuum desiccation for a minimum of 24 hours prior to being derivatized under dried nitrogen using bistrimethyl-silyl-triflouroacetamide (BSTFA)
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