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Tolerant bacterial frequencies were calculated by normalizing c.f.u.s to the initial input
What's the optimal dosing
Its been observed since ancient times by civilizations such as the Babylonians, Greeks, and Romans
The supernatant from the hypotonic solution was combined with the supernatant from the hypertonic solution, centrifuged to remove debris, and dialyzed against 20 mM Tris, pH 7.5 and 150 mM NaCl (Tris-buffered saline, TBS) overnight at 4 C
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