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Techniques including (but not limited to) epithelial cells cultured at the air-liquid interface [227], as well as methods developed by Wine and colleagues [228] and/or Welsh and colleagues [229] are suitable for such studies
B.RamachandranA.LemastersJ
Since peptides, proteins, and hormones can directly reach the brain when administered intranasally via transport and diffusion along the olfactory and trigeminal nerves [241], future studies should explore whether intranasal melatonin could be a viable therapeutic option for increasing brain melatonin levels in individuals at risk of developing AD
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