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Alternatively, glutathione can be quantified after reduction of GSSG in the sample with dithiotreitol (DTT) and subsequent derivatization of GSH with mbrB or mchlB via a reverse-phase high-performance liquid chromatography (HPLC) system equipped with a fluorescent or UV detector, or spectrophotometrically after a GR-mediated glutathione recycling assay (total glutathione) from cell organelles after isolation or fractionation
Dose and cycle length for GI protocols are determined at your provider intake and adjusted based on symptom response
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