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In HPLC analysis, reversed-phase chromatographic columns are usually adopted, with methanol-water or acetonitrile-water as the mobile phase, and semaglutide and its impurities are separated by gradient elution
Wegovy is also a semaglutide medication, like Ozempic, but it's specifically FDA-approved for weight loss rather than for diabetes management
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Sarzi-Puttini P, Giorgi V, Di Lascio S, Fornasari D
Breaking any of them compromises the peptide, reduces potency, and can introduce safety concerns
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