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Finally, they were resuspended in 1 mL of 100 mM Tris solution and the total proteins present in the final samples were quantified using QuantiPro BCA assay (Sigma Aldrich)

[95] Intramuscular or intravenous injection [edit] Injection of hydroxycobalamin is often used if digestive absorption is impaired, [2] but this course of action may not be necessary with high-dose oral supplements (such as 0.51.0 mg or more), [97] [98] because with large quantities of the vitamin taken orally, even the 1% to 5% of free crystalline B 12 that is absorbed along the entire intestine by passive diffusion may be sufficient to provide a necessary amount

Its used to treat and prevent vitamin B12 deficiency anaemia, where you have low levels of this vitamin in your body