Although the actual mechanisms of MTX-induced liver and kidney damage are unknown, one of the hypotheses is the breakdown in cellular antioxidant defences, which increases the production of reactive oxygen species and nitrogen species, inhibits cytosolic NADP-dependent dehydrogenase and NADP malic enzyme, lowers glutathione (GSH), superoxide dismutase (SOD) and catalase levels and reduces the effectiveness of the antioxidant defence system protecting the cell against reactive oxygen species (Reference Savran, Cicek and Doguc7)
This format is especially useful for teams working on NNMT activity, NAD+ pathway analysis, and targeted metabolic signaling studies where a more defined compound profile matters
(2020) Ferroptosis and its potential role in human diseases, Front
(2) Liu JR, Deng ZH, Zhu XJ, Zeng YR, Guan XX, Li JH