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For RNA-seq data analysis, reads of the samples were trimmed for adaptors and low-quality bases using Trimmomatic 0.36 software before alignment with the mouse reference genome (mm10) and the annotated transcripts using STAR 2.5.1

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Crucially, the researchers noted that the S-A-GSH molecule was rapidly deacetylated once inside the body, proving that the acetyl shield successfully protected the compound through the digestive tract and then efficiently released the intact, active glutathione directly into the intracellular environment where it is needed to combat oxidative stress