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ALA's main role in the skin appears to be modulating the immune response of the epidermis

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Cell supernatants and lysates were plated on 7H11 agar medium (Hi Media, Santa Maria, CA, USA) enriched with ADC (GEMINI, USA) and incubated at 37C for three weeks to evaluate mycobacterial growth or survival under treatment conditions by counting the colony-forming units (CFUs)