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The protocol for immobilization of GST-SPINK3 to glutathioneagarose beads was modified from previously reported protocols by using an alternative bifunctional cross-linker (dithiobis(succinimidyl propionate)) in a very simple procedure and by using simple buffers under physiological conditions
For a non-competing adult working under physician supervision, the volume of animal evidence may justify cautious off-label use
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