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Worms were then disrupted using a mortar and pestle for 5 min before centrifuging at 735 g for 5 min
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Overview Detection method- Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm) Species reactivity- Mammalian Applications- The detection sensitivity is ~1 M for the fluorometric assay and ~10 M for the colorimetric assay
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