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Low levels of glutathione are found in deficiencies of key enzymes involved in glutathione metabolism, such as glucose-6-phosphate dehydrogenase, glutathione synthase and glutathione reductase
Notably, overexpression of MANF or treatment with recombinant human MANF could directly reduce A-induced neuronal apoptosis and ER stress [146]
Next, immerse the sections in 0.5% ammonia water for 12 min until the tissue appears distinctly blue
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