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Furthermore, western blot analysis took place by initially preparing cell extracts in a lysis buffer followed by the standard method of ultrasonication

Roszkowska-Gancarz M, Jonas M, Owczarz M, Kurylowicz A, Polosak J, Franek E, Slusarczyk P, Mossakowska M, Puzianowska-Kuznicka M

As the field develops, direct measurement of circulating MOTS-c may become a useful biomarker for guiding dose adjustments, similar to how IGF-1 is used to monitor growth hormone or testosterone levels guide TRT dosing

The reaction mixtures were manually mixed and the fluorescence was recorded on the BioTek H1 Synergy microplate as above