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Since glycolysis is the only way to produce adenosine triphosphate (ATP) and nicotinamide adenine dinucleotide hydrogen (NADH) energy in the RBCs, lactic acid produced in glycolysis leads to a decrease in pH during six weeks of storage

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The chromatographic column can be a C 18 chromatographic column (Kromasil-C 18 , 30mm*250mm, Hanbang Technology Chromatography Co., Ltd.), and the samples are loaded in multiple times, and the effluents of the target peaks are collected respectively

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