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Once opened, store the vial at room temperature and access only with sterile needles using aseptic technique

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Separated proteins were transferred onto Immobilon-P Transfer membranes (MilliporeSigma, IPVH00010), blocked for 1 h using 5% BSA in TBS0.5% Tween-20 and stained overnight with primary rabbit-anti-GCLC (Sigma-Aldrich, HPA036359) or affinity-purified rabbit-anti-GGT1 (GGT129, as previously described 63 ) 1:1,000 in BSA 2.5% overnight at 4 C

It is no coincidence that the quantity of glutathione is greater in the cells of detoxifying organs such as the liver, where it is particularly synthesized and whose action conditions greater control and elimination of excess toxins to which we are constantly exposed and which tend to accumulate in the body