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Researchers utilize DSIP to examine how endogenous peptides coordinate sleep-wake cycles with neuroendocrine stress responses, particularly focusing on its potential to normalize sleep patterns disrupted by stress or circadian disturbances in experimental models
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Total RNA was then used to synthesize complementary DNA, followed by the performance of real-time PCR using an SYBR Green I technology and MxPro-Mx3000P QPCR machine
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