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They can also be produced via various laboratory methods, such as solid-phase peptide synthesis (SPPS)
PTH1R has the longest ECL1, but it is unstructured in both LA-PTHPTH1RG s 19 and ePTHPTH1R 37 structures, probably owing to the great mobility of PTH1Rs ECD and specific binding mode, consistent with the mutagenesis studies
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