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Experimental Results: Cartilage morphology: severe wear, erosion, and fibrosis of cartilage in the control group
The assay involves carefully optimized enzymatic recycling method using glutathione reductase and Ellmans reagent (DTNB)
Animal Toxicology Studies Animal toxicology studies tested doses from 0.0027 mg/lb to 9.1 mg/lb via intraperitoneal, intramuscular, intravenous, and oral routes for up to 6 weeks, finding: No acute toxic or lethal dose No gross or histologic toxicity in major organs No hepatotoxicity or nephrotoxicity Negative Ames test (no mutagenicity) Negative chromosomal aberration tests (no genotoxicity) No teratogenicity in rat pregnancy studies No local injection site irritation The LD1 (minimum lethal dose) could not be achieved despite aggressive testing
The active site of the other three GPXs is cysteine [64]
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