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MCF 10A (breast epithelial, ATCC CRL-10317) were cultured in high glucose Dulbeccos modified Eagles (DMEM) medium and HAMS F12 (1:1 ratio, Euroclone), supplemented with 5% horse serum (Gibco), 1% L-glutamine (Gibco), 1% penicillin/streptomycin (Gibco), 10 g/ml insulin (Sigma), 0.5 g/ml hydrocortisone (Sigma), and 20 ng/ml EGF (Cell Guidance Systems)
doi: 10.1038/nrmicro2070 29 BernardS
Higher Dosage IV Therapy can infuse higher concentration levels of your vitamins and minerals into your body than oral supplements
Chauhan A, Chauhan V, Brown WT, Cohen I
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