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The click reaction was quenched by adding 2 ml cold acetone (80 C), vortexed and stored overnight at 80 C
Failure to increase the number of secreted VLDL particles could indicate suboptimal apoB100 levels as a precipitating factor in NAFLD, and while mRNA levels of apoB100 and MTTP was found to be higher in patients with NAFLD compared to controls [35, 121], apoB100 synthesis rates were lower in patients with NASH ( n = 7) compared to lean ( n = 7) or BMI-matched obese ( n = 7) controls without NASH [122]
