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Once the Opal fluorophore was covalently linked to the antigen, primary and secondary antibodies were removed with a heat retrieval step
Its success in both Ozempic and Wegovy has made it a leading treatment for individuals struggling with obesity or weight-related conditions, providing consistent, reliable results over newer medications like Mounjaro and Zepbound
The tissues were fixed in 4% paraformaldehyde for 48 h at 4 C before sectioning and H&E staining
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