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To further purify FOXO4 FHD and p53 TAD, gel filtration chromatography was conducted using Hi-Load 16/600 superdex 200pg and 75pg (Cytiva) on AKTA pure and AKTA prime systems (Cytiva)
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Spectrophotometric assays measuring nitrite accumulation confirm elevated NO production, with dose-response relationships established across micromolar concentration ranges
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