The diet emphasizes consumption of nutrient-dense whole foods which contain nutrients known to be protective against glutamate excitotoxicity, including foods high in omega-3 fatty acids (86), vitamin C (8789), vitamin D (90), vitamin E (91), riboflavin (vitamin B2) (92, 93), vitamin B6 (94), zinc (95), and magnesium (96, 97)
To stain nuclei of the neurons, the specimens from NG were incubated with Alexa Fluor 488-conjugated Anti-NeuN IgG (Supplementary Table S1) at RT for 1 h, and the specimens from NTS and ARC were incubated overnight with Alexa Fluor 555-conjugated anti-NeuN IgG (Supplementary Table S1) at 4 C
However, do not restrict fluids to the point of dehydration
The dual agonist approach isnt about one magic bullet but rather about comprehensive activation of complementary metabolic pathways