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This difference has a direct consequence for how the protocol is structured
Monitoring mental health during treatment is reasonable
6.6.2 Gut microbiota-mediated biotransformation and mechanistic attribution 6.6.2.1 Microbial metabolic transformation pathways The gut microbiota functions as a metabolic organ, extensively biotransforming natural compounds into secondary metabolites with enhanced bioavailability and biological activity (242)
Desalting was performed for 5 min using 0.1% TFA as eluent with a flow to waste (30 l/min) followed by back-flushing of the sample during the whole analysis from the pre-column to the PepMap100 RSLC C18 nano-HPLC column (2 m, 100 , 75 m ID 50 cm, nanoViper, Dionex, Germany) using a linear gradient starting with 95% solvent A (H 2 O with 0.1% formic acid)/5% solvent B (acetonitrile with 0.1% formic acid) and increasing to 30% solvent B after 35 min (measurements on Q-Exactive Plus) or increasing stepwise to 20% solvent B after 37 min and further to 32% solvent B after 44 min (measurements on Q-Exactive HF) using a flow rate of 300 nl/min
