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After this, we mixed [3- 3 H]-glucose with the non-radioactive glucose infusate (infusate specific activity of 0.5 Ci/mg) during the 2-h clamp period
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Schafer and Buettner (Free Radical Biology and Medicine, 2001) formalised the GSSG/2GSH couple as a quantitative index of the cellular redox environment, a framework still used to describe redox state across proliferation, differentiation and apoptosis in cultured cells
A minor mixing error can disrupt your entire cycle, so lets take it step-by-step and do it correctly
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