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In murine models, Spinacia oleracea -derived CQDs showed safe distribution at concentrations up to 100 g/mL with no histological organ damage, whereas higher doses elicited mild liver lymphocyte infiltration [ Furthermore, cobalt-conjugated N-CQDs demonstrated efficient penetration into neuronal and non-neuronal tissues in zebrafish, a well-established non-mammalian vertebrate model, without eliciting detectable apoptosis or oxidative stress [161, 163]
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Gastrointestinal symptoms have also been reported, including heartburn, abdominal pain, belching, nausea, vomiting, flatulence, constipation, and diarrhea (106)
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