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To examine whether the assembly of Pns11 tubules is dependent on the ATPase activity of Hsp70, an allosteric inhibitor, VER155008, which competes with ATP for Hsp70 binding 39 , was used to inhibit the folding function of Hsp70 in Sf9 cells
Second, the pendant 5-methylpyridine, a motif preserved throughout late-stage optimization, projects deeply into the PDE10A selectivity pocket, forming a critical H-bond with Y683, which is a key contributor to the exceptional isoform selectivity of MK-8189
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