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Human RCC2 was amplified from U-2 OS cDNA and subcloned into a peGFP-C1 vector (Clontech) using restriction digest (SmaI, EcoRI) and Gibson assembly

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Khaydukov EVMironovaKESemchishenVAGeneralovaANNechaev AVKhochenkovDAet al

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Because it is unclear whether biological oxidations and cellular metabolism program directly affect hair cells using this analysis approach, we performed a gene set enrichment analysis (GSEA) using 983 differentially expressed genes (DEGs), which reveals a list of DEGs are enriched in gene ontologies related with appendage development (Supplementary Figure 3b) or positive regulation of canonical Wnt signaling (Fig

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