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Nutritional deficiency concerns specifically: With reduced appetite and smaller meals, people may not meet their micronutrient targets (vitamins A, D, B-complex, minerals like magnesium, zinc, etc)

The GST tag can easily be removed following purification by addition of a protease if a suitable protease-cleavage site has been inserted between the GST-tag and the protein of interest (which is usually included in many commercially available sources of GST-tagged plasmids)

You should seek medical advice if you notice: Severe or persistent symptoms that do not improve with time or simple changes to diet and habits Signs of dehydration such as very dark urine, dizziness or not passing urine for many hours Symptoms that worsen rather than settle after a few days Trust your instincts