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Discussion 1) The study completed by Zhang et al yielded several key findings regarding the role of FOXO4 in Leydig cell senescence and the therapeutic efficacy of FOXO4-DRI in alleviating age-related testosterone insufficiency

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The supernatant was used to detect antioxidant biomarkers, such as the glutathione (GSH), total antioxidant capacity (T-AOC), content and malondialdehyde (MDA) levels, according to the manufacturers instructions (Nanjing Jiancheng Bioengineering Institute, Nanjing, China)

Drugs like NSAIDs and corticosteroids typically used to treat knee OA pain can actually accelerate the condition with long-term use