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Cells were washed once with 1 PBS, and 1,2- 13 C 2 labeled glucose medium was added to the cells for 60 min

Biochemical characterization of glutaredoxins from Chlamydomonas reinhardtii reveals the unique properties of a chloroplastic CGFS-type glutaredoxin

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After thoroughly mixing the extract by vortexing, the precipitated protein was removed by centrifugation (16,000 g , 5 min, 4 C) before the supernatants were analysed by HPLCMS