The combined application of GSH and MT resulted in the best improvement of growth, biomass production and chlorophyll content of Zn-stressed safflower seedlings compared to the single application of GSH or MT
1 H NMR (300 MHz, DMSO) 8.63 (d, J = 5.2 Hz, 2H), 8.09 (d, J = 72.6 Hz, 4H), 7.83 (q, J = 8.2 Hz, 8H), 3.44 (d, J = 4.5 Hz, 4H)
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Free glutathione, GSH, is read first after 15 minutes, followed by addition of a reaction mixture that converts all the oxidized glutathione, GSSG, into free GSH, which then reacts with the excess detection reagent to yield the signal related to Total GSH content