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Cells were harvested, washed once with phosphate buffered saline (PBS, Gibco, #14190-094) and loosened from the cell flasks with Versene (Gibco, #15040-033) or TrypLE TM (Gibco, #12605-010)

For individuals with absorption disorders or pernicious anemia, this direct delivery method is often the preferred route

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doi: 10.2337/DB20-0636 Summary Keywords autoimmunity, coxsackievirus B (CVB), enterovirus, HLA, immune tolerance, stem cell-derived islets, type I interferon (IFN), cells Citation Veronese-Paniagua DA and Millman JR (2025) The perfect storm: unraveling the interplay of genetic predisposition and viral triggers in type 1 diabetes pathogenesis