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Pace the beaker with the liposomal vitamin C into the ultrasonic unit for about 30 minutes
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Unterach, Austria) and the TIR injection (5 mg/0.5 mL) was obtained from Eli Lilly Italy (Sesto Fiorentino 50019, Italy)
Cells were dehydrated with an ethanol series (20%, 50%, 70%, 90% and 100%) on ice for 1 min each and then 100% dry ethanol at room temperature three times for 1 min each, infiltrated with 50:50 dry ethanol:Durcupan ACM resin (Sigma-Aldrich, 44610) for 30 min and then four changes of Durcupan at 1 h each and finally embedded in a vacuum oven at 60 C for 72 h
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