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This increase in natural growth hormone can in turn stimulate the production of insulin-like growth factor-1, or IGF-1

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i) LASSS-treated HGF, at as low as 1 ng/ml that is below 2.510 ng/ml of the optimal final-concentration range in culture media (supplementary Figure 1a, left light-blue bar ), activates satellite cells to the maximum level presented by the positive control ( black bar PC , 5 ng/ml HGF)

The animals were then perfused with cold saline to free them of residual blood, after which liver and other tissues were collected immediately, weighed, quickly diced, and frozen in liquid nitrogen at 80 C