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Melanin, the pigment responsible for skin tone, exists in two forms: blackish-brown eumelanin and reddish-yellow pheomelanin
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The assay plate was incubated for 3 h at 37 C in 5% CO 2 and allowed to equilibrate at room temperature for 5 min after which 30 L SteadyLite Plus (PerkinElmer, 6066759) was added to each well
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