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Eur J Biochem 221:639648 Suthanthiran M, Anderson ME, Sharma VK, Meister A (1990) Glutathione regulates activation-dependent DNA synthesis in highly purified normal human T lymphocytes stimulated via the CD2 and CD3 antigens

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Briefly, Nb35 target gene was transformed in the bacterium and amplified in TB culture medium with 100 g/mL ampicillin, 2 mM MgCl 2 , 0.1 % (w/v) glucose at 37C, 180 rpm