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Brown, Joe Rogan, and Andrew Huberman
Adequate zinc intake is directly relevant: zinc is essential for testosterone synthesis, and deficiency is associated with reduced testosterone levels
Forty-eight hours after transfection, the cells were washed twice in binding buffer (50 mM HEPES, pH 7.4, supplemented with 1 mM CaCl 2 , 5 mM MgCl 2 and 0.5% (w/v) BSA) and incubated for 15 min at 4 C
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