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These experimental results demonstrated that NP4 could activate macrophages, facilitate DCs maturation, increase infiltration of cytotoxic T cells, activate the mouse immune system, and inhibit tumor growth in osteosarcoma

Although physicochemical characterization demonstrated appropriate vesicle size distribution, polydispersity index, zeta potential, and formulation stability, and the increase in vesicle size after RSV incorporation supported the association of RSV with the liposomal system, these parameters do not replace direct quantification of encapsulation efficiency