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Foam on the surface (large, persistent bubbles) indicates you either shook the vial or injected water too aggressively
For cell number measurements in 6-well plates, the medium was aspirated, the wells were rinsed with PBS and the cells were detached by incubating with 500 l of 0.25% trypsin (Thermo Scientific, 25200056) for 20 min at 37 C
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