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Briefly, enzyme activity was determined at 37C in reaction mixtures of 1.0 ml containing 100 mm Tris-HCl buffer (pH 8.2), 150 mm KCl, 5 mm ATP, 2 mm phosphoenolpyruvate, 10 mm glutamate, 10 mm -aminobutyrate, 20 mm MgCl 2 , 2 mm EDTA, 0.2 mm NADH, 17 g pyruvate kinase, and 17 mg lactate dehydrogenase

Why Trust Everyday Health Caitlin Beale, MS, RDN Author Caitlin Beale, MS, RDN, is a registered dietitian and freelance health writer with experience in clinical nutrition, education, research, and private practice