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For analysis of miRNAs, cDNA was synthesized from 10 ng of DNase Itreated total RNA using the TaqMan microRNA reverse transcription kit (Applied Biosystems PN4366596) with RT primers from TaqMan sets for miR18a (Cat #002422), and snoRNA202 (Cat #4427975) as endogenous control
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On the contrary, Ang-2 and -3 can competitive combined TIE2 without stimulating tyrosine phosphorylation, which stopping the signal transduction of angiogenesis (Sallinen et al., 2010
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