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The expression of target genes was analysed via quantitative real-time PCR with a QuantaStudio 5 qPCR machine (Applied Biosystems, Thermo Fisher Scientific)
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While male ghrelin knockout mice show an attenuated hyperlocomotion, NAc-dopamine and rCPP after alcohol (74), this alcohol-induced activation of the mesolimbic dopamine system is unaltered by neutralization of circulating ghrelin (70)
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