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(2020) found that there was no association of sKlotho with CAC or AVC by multivariate analysis, although the sKlotho level in patients with stage-5 CKD was dramatically reduced in comparison with that in patients with stage-3 CKD (Savvoulidis et al., 2020)
2.6 Microbial bioinformatics analysis Microbial bioinformatics analysis was performed as previously described ( 2.7 Preparation of serum samples for mass spectrometry analysis Serum samples were thawed on ice, and 100 L of each sample was mixed with 300 L of precooled methanol and acetonitrile (2:1, v/v)
de Castro, J
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